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Worthington組織消化指南
引言
組織消化酶主要用于組織培養(yǎng)和細(xì)胞生物學(xué)研究的原代細(xì)胞的分離和收集。盡管這些消化酶已經(jīng)普遍應(yīng)用多年，但是對(duì)其組織消化和細(xì)胞收集的作用機(jī)制卻沒(méi)有全面的理解。 由此導(dǎo)致的結(jié)果便是選擇一種消化方法大多是隨機(jī)選擇或基于過(guò)去的經(jīng)驗(yàn)，而不是在*理解消化原理的基礎(chǔ)上選擇的消化方案，以及如何根據(jù)具體實(shí)驗(yàn)?zāi)康男拚桨敢垣@得更好的實(shí)驗(yàn)結(jié)果。
細(xì)胞分離程序的目的是zui大程度的獲得功能活性的單個(gè)細(xì)胞，而影響結(jié)果的原因有很多，包括（不限于）以下：
1、組織類型
2、物種來(lái)源
3、動(dòng)物年齡
4、遺傳改造情況（如knockouts）
5、所用的消化培養(yǎng)液
6、所用的酶
7、消化酶中的雜質(zhì)
8、所用消化酶的工作濃度
9、溫度
10、孵育時(shí)間
通常前四個(gè)原因沒(méi)辦法選擇，而剩下的原因一般由經(jīng)驗(yàn)決定以獲取zui適實(shí)驗(yàn)結(jié)果。
通過(guò)查閱文獻(xiàn)來(lái)選擇*組織消化酶和zui適消化條件的研究人員通常被互相矛盾的實(shí)驗(yàn)數(shù)據(jù)搞得一頭霧水。這些巨大差異來(lái)源于組織胞外基質(zhì)的復(fù)雜而動(dòng)態(tài)的特性，以及過(guò)去常用的粗制、未提純的組織分離酶。另外，組織胞外基質(zhì)由各種蛋白、糖蛋白、脂類和糖脂組成，這些成分的組成比例在不同物種、不同組織、甚至不同發(fā)育（年齡）之間存在明顯的差異，這些因素都極大的影響了消化方案的制定。目前通常使用的粗制酶，如鏈霉蛋白酶、NF1:250、以及含有幾種其他不同濃度蛋白酶的膠原酶、以及各種多糖酶、核酶和脂酶。
本指南總結(jié)了目前常用組織消化酶的zui適消化和細(xì)胞收集知識(shí)和方法，描述了標(biāo)準(zhǔn)的實(shí)驗(yàn)步驟， 以便于制定一個(gè)*的細(xì)胞分離方案，同時(shí)列舉了一些相關(guān)的參考文獻(xiàn)。
下表列出多種組織的適宜消化酶（含參考文獻(xiàn)，以組織類型和物種分類，詳見(jiàn)鏈接）
脂肪組織 | 腎上腺組織 | 骨組織 | 腦組織 |
軟骨組織 | 結(jié)腸組織 | 內(nèi)皮組織 | 上皮組織 |
眼 | 心臟 | 腸 | 腎 |
肝 | 肺 | 淋巴結(jié) | 乳腺 |
其它組織 | 其他組織 | 肌組織 | 神經(jīng)組織 |
胰腺 | 腮腺 | 垂體 | 前列腺 |
生殖 | 動(dòng)物鱗片 | 皮膚 | 脾臟 |
干細(xì)胞 | 胸腺 | 甲狀腺/甲狀旁腺 | 扁桃體 |
腫瘤 |
|
|
|
注：使用Worthington公司消化酶的文獻(xiàn)不限于上表鏈接中所列，一般而言，Worthington公司的組織消化酶可用于大多組織類型的消化。
心臟組織消化手冊(cè)
| 物種 | 詳細(xì)來(lái)源 | 細(xì)胞名稱 | 消化用酶 | 培養(yǎng)基 | 參考文獻(xiàn) |
| 牛 | 牛 | Heart | Trypsin: 0.06% | 25mM HEPES buffered Locke's solution, CMF | Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and B綿羊 Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990 |
| (包括大鼠) | Adrenal chromaffin | ||||
| Paraneurons | |||||
| 犬 | 犬 | Cardiomyocytes | Collagenase Type 2: 150 u/ml | Tyrode's solution | Zhang,D., Wu, C., Qi, X., Meijering, R., Hoogstra-Berends, F., Tadevosyan, A., Cubukcuoglu, et al: Activation of Histone Deacetylase-6 Induces Contractile Dysfunction Through Derailment of alpha-tubulin Proteostasis in Experimental and Human Atrial Fibrillation., Circulation 129, 346-58, 2014 |
| 犬 | 犬, adult | Atrial and Ventricular Myocytes | Collagenase Type 2: 0.065% | See reference | Bonilla, I., Sridhar, A., Nishijima, Y., Gyorke, S., Cardounel, A. and Carnes, C.: Differential Effects of the Peroxynitrite Donor, SIN-1, on Atrial and Ventricular Myocyte Electrophysiology., J Cardiovasc Pharmacol 61, 401-7, 2013 |
| 犬 | 犬 | Cardiomyocytes | Collagenase Type 2: 300 u/ml | Tyrode's solution | Xi, Y., Wu, G., Ai, T., Cheng, N., Kalisnik, J., Sun, J., Abbasi, S., Yang, D., Fan, C., Yuan, X., Wang, S. et al: Ionic Mechanisms Underlying the Effects of Vasoactive Intestinal Polypeptide on Canine Atrial Myocardium., Circ Arrhythm Electrophysiol 6, 976-83, 2013 |
| Protease: 0.03% | |||||
| 犬 | 犬 | Atrial Myocytes | Collagenase Type 2: 0.03% | Tyrode's solution | Gan, T., Qiao, W., Xu, G., Zhou, X., Tang, B., Song, J., Li, Y., Zhang, J., Li, F., Mao, T. and Jiang, T.: Aging-Associated Changes in L-type Calcium Channels in the Left Atria of Dogs., Exp Ther Med 6, 919-924, 2013 |
| 犬 | 犬 | Cardiomyocytes | Collagenase Type 2: 60 u/ml | See reference | Harleton, E., Besana, A., Comas, G., Danilo, P., Rosen, T., Argenziano, M., Rosen, M., Robinson, R. and Feinmark, S.: Ability to Induce Atrial Fibrillation in the Peri-Operative Period is Associated with Phosphorylation-Dependent Inhibition of TWIK Protein-Related Acid-Sensitive Potassium Channel 1 (TASK-1)., J Biol Chem 288, 2829-38, 2013 |
| Protease XIV: 0.5 u/ml | |||||
| 犬 | 犬 | Atrial and Ventricular Myocytes | Collagenase Type 2: 0.05-0.08% | See reference | Zhang, H., Silva, J., Lin, Y., Verbsky, J., Lee, U., Kanter, E., Yamada, K., Schuessler, R. and Nichols, C.: Heterogeneity and Function of K(ATP) Channels in Canine Hearts., Heart Rhythm 10, 1576-83, 2013 |
| 犬 | 犬, adult | Cardiomyocytes | Collagenase Type 2: 0.05% | HEPES | Calloe, K., Nof, E., Jespersen, T., Di Diego, J., Chlus, N., Olesen, S.? Antzelevitch, C. and Cordeiro, J.: Comparison of the Effects of a Transient Outward Potassium Channel Activator on Currents Recorded from Atrial and Ventricular Cardiomyocytes., J Cardiovasc Electrophysiol 22, 1057-66, 2011 |
| Protease Type XIV: 0.01% | |||||
| 犬 | 犬, 20-25 kg | Cardiomyocytes | Collagenase Type 2: 0.05% | Tyrode's solution | Burashnikov, A., Zygmunt, A., Di Diego, J., Linhardt, G., Carlsson, L. and Antzelevitch, C.: AZD1305 Exerts Atrial Predominant Electrophysiological Actions and is Effective in Suppressing Atrial Fibrillation and Preventing its Reinduction in the Dog., J Cardiovasc Pharmacol 56, 80-90, 2010 |
| 犬 | 犬, adult | Cardiomyocytes | Collagenase Type 2: 0.05% | M199 | Gavi, S., Yin, D., Shumay, E., Wang, H. and Malbon, C.: Insulin-Like Growth Factor-I Provokes Functional Antagonism and Internalization of Beta1-Adrenergic Receptors., Endocrinology 148, 2653-62, 2007 |
| Protease: 0.008% | |||||
| 犬 | 犬 | Atrial and Ventricular myocytes | Collagenase Type 2: 0.065% | Tyrode's solution | Schotten, U., de Haan, S., Verheule, S., Harks, E., Frechen, D., Bodewig, E., Greiser, M., Ram, R., Maessen, J., Kelm, M., Allessie, M. and Van Wagoner, D.: Blockade of Atrial-Specific K+-currents Increases Atrial but not Ventricular Contractility by Enhancing Reverse Mode Na+/Ca2+-Exchange.,Cardiovasc Res 73, 37-47, 2007 |
| 犬 | 犬 | Atrial myocytes | Collagenase Type 2: 0.013% | HEPES | Baba, S., Dun, W., Hirose, M. and Boyden, P.: Sodium Current Function in Adult and Aged Canine Atrial Cells., Am J Physiol Heart Circ Physiol 291, H756-61, 2006 |
| 犬 | 犬, 20-30 kg | Cardiomyocytes | Collagenase Type 2: 110 u/ml | Tyrode's solution | Sun, H., Chartier, D., Leblanc, N. and Nat, S.: Intracellular Calcium Changes and Tachycardia-Induced Contractile Dysfunction in Canine Atrial Myocytes., Cardiovasc Res 49, 751-61, 2001 |
| 犬 | 犬 | Ventricular and Atrial Myocytes | Collagenase Type 4: 0.04% | HEPES/Tyrode's | Gintant, G.: Two Components of Delayed Rectifier Current in Canine Atrium and Ventricle. Does IKs Play a Role in the Reverse Rate Dependence of Class III Agents?, Circ Res 78, 26-37, 1996 |
| Collagenase Type 2: 125 u/ml | |||||
| 犬 | Dog, mongrel, adult | Myocytes | Hyaluronidase: 0.1% | Joklik's MEM | Spanier, A. and Weglicki, W.: Ca2+-Tolerant Adult Canine Myocytes: Preparation and Response to Anoxia/Acidosis, Am J Physiol 243, H448, 1982 |
| 雞 | 雞, embryonic | Cardiomyocytes | Trypsin: 0.13% | HBSS | Blech-Hermoni, Y. and Ladd, A.: Identification of Transcripts Regulated by CUG-BP, Elav-like Family Member 1 (CELF1) in Primary Embryonic Cardiomyocytes by RNA-seq., Genom Data 6, 74-76, 2015 |
| Collagenase Type 2: 0.13% | |||||
| Deoxyribonuclease I: 0.033% | |||||
| 雞 | 雞 embryos, 9-11 day incubated | Cardiomyocytes | Trypsin: 0.25% | PBS | Eschenhagen, T., Fink, C., Remmers, U., Scholz, H., Wattchow, J., Weil, J., et al.: Three-Dimensional Reconstitution of Embryonic Cardiomyocytes in a Collagen Matrix: A New Heart Muscle Model System, FASEB J 11, 683, 1997 |
| 雞 | 小雞, embryos, 10-11 day | Cardiomyocytes | Trypsin: 0.17% | HBSS, CMF | Wang, S., Greaser, M.L., Schultz, E., Bulinski, J.C., Lin, J.J., and Lessard, J.L.: Studies on Cardiac Myofibrillogenesis With Antibodies to Titin, Actin, Tropomyosin, and Myosin, J Cell Biol 107, 1075, 1988 |
| 雞 | 小雞, 10 day | Ventricular | Trypsin: 0.025% | HBSS, CMF | Kim, D., Okada, A., and Smith, T.W.: Control of Cytosolic Calcium Activity During Low Sodium Exposure in Cultured Chick Heart Cells, Circ Res 61, 29, 1987 |
| 雞 | 小雞, embryo, 11 day | Cardiomyocytes | Trypsin: 0.05% | HBSS, CMF | Jacob, R., Lieberman, M., Murphy, E., and Piwnica-Worms, D.: Effect of Sodium-Potassium Pump Inhibition and Low Sodium on Membrane Potential in Cultured Embryonic Chick Heart Cells, J Physiol 387, 549, 1987 |
| 雞 | 小雞, embryonic, 11-12-day-old | Myocytes | Trypsin: 0.025% | CMF solution | Murphy, E., Aiton, J., Russell, C., and Lieberman, M.: Calcium Elevation in Cultured Heart Cells: Its Role in Cell Injury, Am J Physiol Cell Physiol 245 (14), C316, 1983 |
| 雞 | 小雞, 8 day embryo | Myocytes | Trypsin NF 1:250: 0.25% | Rinaldini's buffer solution, CF | Gross, W., Schopf-Ebner, E., and Bucher, O.: Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells, Exp Cell Res 53, 1, 1968 |
| (also rat, neonate and mouse) | |||||
| 雞 | Hen, White Leghorn | Heart | Trypsin: 0.025% | Medium 199 | Dehann, R.: Regulation of Spontaneous Activity and Growth of Embryonic Chick Heart Cells in Tissue Culture, Dev Biol 16, 216, 1967 |
| 雞 | 小雞embryos, 5 day | Heart | Trypsin: 3.0% | Tyrode's solution, CMF | Steinberg, M.: "ECM": Its Nature, Origin, And Function in Cell Aggregation, Exp Cell Res 30, 257, 1963 |
| Liver | |||||
| 貓科動(dòng)物 | 貓, mongrel, adult, 1.8-2.8 Kg | Myocytes | Collagenase Type 2: 0.12% | Kreb's Henseleit, CF | Silver, L., Hemwall, E., Marino, T., and Houser, S.: Isolation and Morphology of Calcium-Tolerant Feline Ventricular Myocytes, Am J Physiol 245, H891, 1983 |
| Ventricular | |||||
| 魚(yú) | 魚(yú), 斑馬魚(yú) | Cardiomyocytes | Collagenase Type 2: 0.5% | MEM | Sander, V., Sune, G., Jopling, C., Morera, C. and Belmonte J.: Isolation and In Vitro Culture of Primary Cardiomyocytes from Adult Zebrafish Hearts., Nat Protoc 8, 800-9, 2013 |
| Collagenase Type 4: 0.5% | |||||
| 青蛙 | 青蛙 (Rana esculenta) | Myocytes | Trypsin: 0.04% | CF Ringer | Arrio-Dupont, M., and de Nay, D.: High Yield Preparation of Calcium-Tolerant Myocytes From Frog Ventricles, Biol Cell 54, 164, 1985 |
| 豚鼠 | Guinea pig, adult | Cardiomyocytes | Collagenase Type 2: 100 u/ml | M-199 | Zorn-Pauly K, Schaffer P, Pelzmann B, Bernhart E, Lang P, and Koidl B: L-type and T-type Ca2+ current in cultured ventricular guinea pig myocytes,Physiol Res 53(4), 369, 2004 |
| 豚鼠 | 豚鼠 | Cardiomyocytes | Collagenase Type 2: 100-200 u/ml | See reference | Dhamoon, A, Pandit, S., Sarmast, F., Parisian, K., Guha, P., Li, Y., Bagwe, S., Taffet, S. and Anumonwo, J.: Unique Kir2.x Properties Determine Regional and Species Differences in the Cardiac Inward Rectifier K+ Current.,Circ Res 94, 1332-9, 2004 |
| 豚鼠 | Guinea pig, female, 300-350g | Coronary endothelial | Collagenase Type 2: 0.1% | see reference | Buxton I L, Kaiser R A, Oxhorn B C, Cheek D J: Evidence supporting the Nucleotide Axis Hypothesis: ATP release and metabolism by coronary endothelium, Am J Physiol Heart Circ Physiol 281, H1657-66, 2001 |
| 豚鼠 | Guinea pig, 250-450 g | Endothelial | Collagenase Type 2: 0.15% | Perfusing solution | Preisig-Muller, R., Mederos Y Schnitzler, M., Derst, C., and Daut J.:Separation of Cardiomyocytes and Coronary Endothelial Cells for Cell-Specific RT-PCR, Am J Physiol 277, H413, 1999 |
| (see reference) | |||||
| 豚鼠 | 豚鼠, male, 450-600 g | Myocytes | Hyaluronidase: 0.02% | Krebs Henseleit bicarbonate buffer | Stemmer, P., Akera,T., Brody, T., Rardon, D., and Watanabe, A.: Isolation and Enrichment of Ca2+-Tolerant Myocytes for Biochemical Experiments from Guinea-Pig Heart, Life Sci 44, 1231, 1989 |
| 豚鼠 | 豚鼠 | Cardiomyocytes | Collagenase: 0.04% | Tyrode solution, CF | Ishihara, K., Mitsuiye, T., Noma, A., and Takano, M.: The Mg2+ Block and Intrinsic Gating Underlying Inward Rectification of the K+ Current in Guinea-Pig Cardiac Myocytes, J Physiol 419, 297, 1989 |
| 豚鼠 | 豚鼠 | Myocytes | Hyaluronidase: 0.10% | Bicarbonate buffer, CF | Bridge, J., Spitzer, K., and Ershler, P.: Relaxation of Isolated Ventricular Cardiomyocytes by a Voltage-Dependent, Science 241, 823, 1988 |
| 人類 | 人類 | Cardiac | Collagenase: 0.045% | DPBS | Holt-Casper, D., Theisen, J., Moreno, A., Warren, M., Silva, F., Grainger, D., Bull, D. and Pa, A.: Novel Xeno-free Human Heart Matrix-Derived Three-Dimensional Scaffolds., J Transl Med 13, 194, 2015 |
| Pancreatin: 0.1% | |||||
| 人類 | 人類 | Cardiac stem | Collagenase Type 2: 0.25% | Joklik modified Eagle抯 | Avolio, E., Gianfranceschi, G., Cesselli, D., Caragnano, A., Athanasakis, E., Katare, R., Meloni, M., Palma, A., Barchiesi, A., Vascotto, C., Toffoletto, B., Mazzega, E., Finato, N., Aresu, G. and Livi, U.: Ex vivo Molecular Rejuvenation Improves the Therapeutic Activity of Senescent Human Cardiac Stem Cells in a Mouse Model of Myocardial Infarction., Stem Cells 32, 2373, 2014 |
| 人類 | 人類 | Atrial myocytes | Collagenase Type 1: 286 u/ml | see references | Voigt, N., Zhou, X. and Dobrev, D.: Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents., J Vis Exp , e50235, 2013 |
| Protease: 5 u/ml | |||||
| 人類 | 人類 | Myofibers | Collagenase Type 1: 0.3% | see reference | Anderson, E., Rodriguez, E., Anderson, C., Thayne, K., Chitwood, W. and Kypson, A.: Increased Propensity for Cell Death in Diabetic Human Heart is Mediated by Mitochondrial-Dependent Pathways., Am J Physiol Heart Circ Physiol 300, H118, 2011 |
| 人類 | 人類 | Atrial cardiomyocytes | Collagenase Type 1: 286 u/ml | see reference | Voigt, N., Makary, S., Nat, S. and Dobrev, D.: Voltage-Clamp-Based Methods for the Detection of Constitutively Active Acetylcholine-Gated I(K,ACh) Channels in the Diseased Heart., Methods Enzymol 484, 653, 2010 |
| Protease: 5 u/ml | |||||
| 人類 | 人類 | Coronary artery smooth muscle | Collagenase Type 2: 0.1% | DMEM | Jensen, B., Swigart, P., Laden, M., DeMarco, T., Hoopes, C. and Simpson, P.: The Alpha-1D Is the Predominant Alpha-1-Adrenergic Receptor Subtype in Human Epicardial Coronary Arteries., J Am Coll Cardiol 54, 1137, 2009 |
| Elastase: 0.05% | |||||
| 人類 | 人類 | Smooth muscle aortic | Collagenase Type 1: 0.2% | DMEM | Mathew, S., Tustison, K., Sugatani, T., Chaudhary, L., Rifas, L. and Hruska, K.: The Mechanism of Phosphorus as a Cardiovascular Risk Factor in CKD., J Am Soc Nephrol 19, 1092, 2008 |
| Collagenase Type 2: 0.1% | |||||
| Elastase: 0.025% | |||||
| 人類 | 人類 (n=16, age: 60 &plusmin;3 years) | Myocardial | Hyaluronidase: 0.05% | Medium 199 | Mukerjee, R., Multani, M., Sample, J., Dowdy, K., Zellner, J., Hoover, D., and Spinale, F.: Effects of adrenomedullin on human myocyte contractile function and beta-adrenergic response, J Cardiovasc Pharmacol 7 (4), 235, 2002 |
| 人類 | 人類 | Cardiomyocytes | Collagenase Type 2: 0.05% | HEPES solution | Todor Anastassia, Sharov Victor G, Tanhehco Elaine J, Silverman Norman, Bernabei Alvise, Sabbah Hani N: Hypoxia-induced cleavage of caspase-3 and DFF45/ICAD in human failed cardiomyocytes, Am J Physiol Heart Circ Physiol 283, H990-5, 2002 |
| Collagenase Type 1: 0.025% | |||||
| Protease XIV: 0.013% | |||||
| 人類 | 人類 | Heart | Collagenase Type 2: 200 u/ml | Tyrode's solution | Hoppe, U., Jansen, E., Sudkamp, M., and Beukelmann, D.:Hyperpolarization-Activated Inward Current in Ventricular Myocytes From Normal and Failing Human Hearts, Circulation 97, 55, 1998 |
| Myocytes | |||||
| 人類 | 人類 | Atrial myocytes | Collagenase Type 2: 0.1% | see reference | Van Wagoner, D., Pond, A., McCarthy, P., Trimmer, J. and Nerbonne, J.:Outward K+ Current Densities and Kv1.5 Expression are Reduced in Chronic Human Atrial Fibrillation., Circ Res 80, 772-81, 1997 |
| Protease Type XIV: 0.04% | |||||
| 人類 | 人類, fetal, 14.5 week gestation | Myocytes | Hyaluronidase: 0.1% | HBSS with Calcium | Goldman, B., and Wurzel, J.: Effects of Subc*tion and Culture Medium on Differentiation of Human Fetal Cardiac Myocytes, In Vitro Cell Dev Biol 28, 109, 1992 |
| 人類 | 人類, both sexes, 18-81 years | Myocardial | Collagenase: 0.14% | HBSS, CMF | Smith, D., Glover, J., Townsend, L., and Maupin, E.: A Method for the Harvest, Culture, and Characterization of Human Adult Atrial Myocardial Cells: Correlation with Age of Donor, In Vitro Cell Dev Biol 27, 914, 1991 |
| Atrial | |||||
| 人類 | 人類, adult | Myocytes | Trypsin: 0.25% | Joklik's MEM | Bugaisky, L.B.: Biology of Isolated Adult Cardiac Myocytes,Isolation and Culture of Human Adult Cardiac Myocytes,(Clark,Decker,Borg,eds), Elsevier Science Publishing Co., Inc., , 1988 |
| 人類 | 人類 | Thoracic aorta | Collagenase: 0.2% | Phosphate buffer w/NaCl | Hassler, O., Wiren, M., and Herbertsson, S.: The Elastic Coat of the Arterial Wall Studied with the Aid of Collagenase, Acta Pathol Microbiol Scand 57, 15, 1963 |
| 無(wú)脊椎動(dòng)物 | E. cirhosa章魚(yú), 260-352 克 | Systemic heart cardiomyocytes | Collagenase: 0.025% | see reference | Altimiras J., Hove-Madsen L., and Gesser H.: Ca(2+) Uptake in the Sarcoplasmic Reticulum from the Systemic Heart of Octopod Cephalopods, J Exp Biol 202, 2531, 1999 |
| Trypsin: 0.02% | |||||
| 小鼠 | 小鼠, 15-30 gm | Ventricular myocytes | Collagenase Type 2: 0.12% | see reference | Henn, M., Janjua, M., Kanter, E., Makepeace, C., Schuessler, R., Nichols, C. and Lawton, J.: Adenosine Triphosphate-Sensitive Potassium Channel Kir Subunits Implicated in Cardioprotection by Diazoxide., J Am Heart Assoc 4, , 2015 |
| 小鼠 | 小鼠, 3-4 week | Cardiac progenitor | Collagenase Type 1: 0.1% | DMEM | Wang, H., Chen, H., Feng, B., Wang, X., He, X., Hu, R., Yin, M., Wang, W., Fu, W. and Xu, Z.: Isolation and Characterization of a Sca-1+/CD31- Progenitor Cell Lineage Derived From Mouse Heart Tissue., BMC Biotech4, 75, 2014 |
| 小鼠 | 小鼠, adult | Cardiac Progenitor | Collagenase Type 2: 600 u/ml | MEM | Santos Nascimento, D, Mosqueira, D, Sousa, L, Teixeira, M, Filipe, M, Resende, T, Araujo, A, Valente, M, Almeida, J, Martins, J, Santos, J, Barcia, R, Cruz, P, Cruz, H and Pinto-d-O, P: Human Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Attenuate Remodeling after Myocardial Infarction by Proangiogenic, Antiapoptotic, and Endogenous Cell-Activation Mechanisms., Stem Cell Res Ther 5, 5, 2014 |
| Deoxyribonuclease I: 60 u/ml | |||||
| 小鼠 | 小鼠 | Cardiac progenitor | Collagenase Type 2: 0.1% | HBSS | Zafiriou, M., Noack, C., Unsold, B., Didie, M., Pavlova, E., Fischer, H., Reichardt, H., Bergmann, M., El-Armouche, A., Zimmermann, W., Zelarayan, L.: Erythropoietin Responsive Cardiomyogenic Cells Contribute to Heart Repair Post Myocardial Infarction., Stem Cells 32, 2480, 2014 |
| Neutral Protease: 2.4 u/ml | |||||
| 小鼠 | 小鼠, adult | Cardiomyocytes | Collagenase Type 2: 0.08% | see reference | Kohncke, C. Lisewski, U., Schleussner, L., Gaertner, C., Reichert, S. and Roepke, T.: Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes., J Vis Exp 73, e50145, 2013 |
| 小鼠 | 小鼠 | Cardiomyocytes | Collagenase Type 2: 150 u/ml | MEM | Hennessey, J., Wei, E. and Pitt, G.: Fibroblast Growth Factor Homologous Factors Modulate Cardiac Calcium Channels., Circ Res 113, 381-8, 2013 |
| 小鼠 | 小鼠 | Cardiomyocytes | Collagenase Type 2: 360 u/ml | L-15 | Touchberry, C., Green, T., Tchikrizov, V., Mannix, J., Mao, T., Carney, B., Girgis, M., Vincent, R., Wetmore, L., Dawn, B., Bonewald, L., Stubbs, J. and Wacker, M.: FGF23 is a Novel Regulator of Intracellular Calcium and Cardiac Contractility in Addition to Cardiac Hypertrophy., Am J Physiol Endocrinol Metab 304, E863, 2013 |
| Papain: 0.4 u/ml | |||||
| Deoxyribonuclease I: 40 u/ml | |||||
| 小鼠 | 小鼠, 2-6 month | Sinoatrial node | Collagenase Type 2: 229 u/ml | Tyrode's solution | Chris, C., Cardona, N., Mesirca, P., Herrmann, S., Hofmann, F., Striessnig, J., Ludwig, A., Mangoni, M. and Lee, A.: Distinct Localization and Modulation of Cav1.2 and Cav1.3 L-type Ca2+ Channels in Mouse Sinoatrial Node., J Physiol 590, 6327, 2012 |
| Elastase: 1.9 u/ml | |||||
| Protease Type XIV: 0.9 u/ml | |||||
| 小鼠 | 小鼠, male, 8-25 week | Cardiomyocytes | Collagenase Type 2: 59 u/ml | MEM | Carley, A. and Kleinfeld, A.: Fatty Acid (FFA) Transport in Cardiomyocytes Revealed by Imaging Unbound FFA is Mediated by an FFA Pump Modulated by the CD36 Protein., J Biol Chem 286, 4589-97, 2011 |
| 小鼠 | 小鼠 | Aortic adventitial leukocytes | Collagenase Type 2: 300 u/ml | PBS | Butcher, M., Herre, M., Ley, K. and Galkina, E.: Flow Cytometry Analysis of Immune Cells Within Murine Aortas., J Vis Exp 53, e2828, 2011 |
| Elastase: 5.6 u/ml | |||||
| 小鼠 | 小鼠, adult | Cardiomyocytes | Collagenase Type 1: 0.1-0.4% | see reference | Flynn, J., Santana, L. and Melov, S.: Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes., J Vis Exp 58, e3302, 2011 |
| Protease Type XIV: 0.004-.02% | |||||
| 小鼠 | 小鼠, male, 6 week | Coronary endothelial cells | Collagenase Type 2: 0.1% | M199 | Makino, A., Suarez, J., Wang, H., Belke, D., Scott, B. and Dillmann, W.:Thyroid Hormone Receptor-beta is Associated With Coronary Angiogenesis During Pathological Cardiac Hypertrophy., Endocrinology 150, 2008, 2009 |
| Neutral Protease: 0.6 u/ml | |||||
| 小鼠 | 小鼠, adult | Ventricular myoctes | Collagenase: 0.1% | M199 | Zhang, Y, Kanter, E., Laing, J., Aprhys, C., Johns, D., Kardami, E. and Yamada, K.: Connexin43 Expression Levels Influence Intercellular Coupling and Cell Proliferation of Native Murine Cardiac Fibroblasts., Cell Commun Adhes 15, 289, 2008 |
| 小鼠 | 小鼠, adult, 9-13 week | Cardiomyocytes | Collagenase Type 2: 620 u/ml | Myocyte buffer see reference | Kabaeva, Z., Zhao, M. and Michele, D.: Blebbistatin Extends Culture Life of Adult Mouse Cardiac Myocytes and Allows Efficient and Stable Transgene Expression., Am J Physiol Heart Circ Physiol 294, H1667-74, 2008 |
| Protease XIV: 0.104 u/ml | |||||
| Deoxyribonuclease I: 0.0015% | |||||
| 小鼠 | 小鼠, adult | Cardiomyocytes | Collagenase: see reference | HBSS | O'Connell Timothy D, Rodrigo Manoj C, Simpson Paul C: Isolation and culture of adult mouse cardiac myocytes, Methods Mol Biol 357, 271-96, 2007 |
| 小鼠 | 小鼠 | Smooth muscle cells | Collagenase Type 2: 300 u/ml | F10 Ham | Verheye, S., Martinet, W., Kockx, M., Knaapen, M., Salu, K., Timmermans, J., Ellis, J., Kilpatrick, D., DeMayer, G.: Selective Clearance of Macrophages in Atherosclerotic Plaques by Autophagy, J Am Coll Cardiol49, 706, 2007 |
| Elastase: 5 u/ml | |||||
| 小鼠 | 小鼠, adult, 6-12 week | Cardiomyocytes | Collagenase Type 2: 0.1% | see reference | Shioya, T: A Simple Technique for Isolating Healthy Heart Cells from Mouse Models., J Physiol Sci 57, 327-35, 2007 |
| Trypsin: 0.006% | |||||
| Protease XIV: 0.006% | |||||
| 小鼠 | 小鼠 | Ventricular myocytes and mesenteric arterial SMC | Collagenase Type 2: 0.06% | Krebs-Ringer | Lu Tong, Ye Dan, Wang Xiaoli, Seubert John M, Graves Joan P, Bradbury J Alyce, Zeldin Darryl C, Lee Hon-Chi: Cardiac and vascular KATP channels in rats are activated by endogenous epoxyeicosatrienoic acids through different mechanisms, J Physiol 575, 627-44, 2006 |
| Papain: 0.175% | |||||
| 小鼠 | 小鼠 | Cardiomyocytes | Collagenase Type 1: 0.17% | PBS | Chen, H., Yong, W., Ren, S., Shen, W., He, Y., Cox, K., Zhu, W., Li, W., Soonpaa, M., Payne, RM, Franco, D., Field, L., Rosen, V., Wang, Y. and Shou, W.: Overexpression of Bone Morphogenetic Protein 10 in Myocardium Disrupts Cardiac Postnatal Hypertrophic Growth., J Biol Chem 281, 27481, 2006 |
| 小鼠 | 小鼠, neonatal | Cardiomyoctes | Collagenase Type 1: 150 u/ml | M199 | Takahashi N, Wang X, Tanabe S, Uramoto H, Jishage K, Uchida S, Sasaki S, Okada Y.: ClC-3-independent sensitivity of apoptosis to Cl- channel blockers in mouse cardiomyocytes, Cell Physiol Biochem 15, 263, 2005 |
| Trypsin: 0.01% | |||||
| 小鼠 | 小鼠, 1 day | Cardiomyoctes | NCIS kit: per instructions | EBSS | Potts, Malia B., Vaughn, Allyson E., McDonough, Holly, Patterson, Cam, and Deshmukh, Mohanish: Reduced Apaf-1 levels in cardiomyocytes engage strict regulation of apoptosis by endogenous XIAP, J Cell Biol 171, 925, 2005 |
| 小鼠 | 小鼠 | Aortic endothelial | Collagenase Type 2: 0.2% | DMEM | Kobayashi, M., Inoue, K., Warabi, E., Minami, T. and Kodama, T.: A Simple Method of Isolating Mouse Aortic Endothelial Cells., J Atheroscler Thromb 12, 138, 2005 |
| 小鼠 | 小鼠 | Smooth muscle cells | Collagenase Type 1: 0.1% | DMEM | Fukumoto Yoshihiro, Deguchi Jun-o, Libby Peter, Rabkin-Aikawa Elena, Sakata Yasuhiko, Chin Michael T, Hill Christopher C, Lawler Patrick R, Varo Nerea, Schoen Frederick J, Krane Stephen M, Aikawa Masanori:Genetically determined resistance to collagenase action augments interstitial collagen accumulation in atherosclerotic plaques, Circulation 110, 1953-9, 2004 |
| Elastase: 0.0125% | |||||
| 小鼠 | 小鼠, female, 14-18 g | Cardiomyocytes | Collagenase Type 2: 0.02% | see reference | Zhang Sui, Wang Dachun, Estrov Zeev, Raj Sean, Willerson James T, Yeh Edward T H: Both cell fusion and transdifferentiation account for the transformation of human peripheral blood CD34-positive cells into cardiomyocytes in vivo, Circulation 110, 3803-7, 2004 |
| Elastase: 0.03% | |||||
| Pancreatin: 0.06% | |||||
| 小鼠 | 小鼠, 6-8 week | Vascular smooth muscle | Papain: 10 u/ml | EBSS | Qian, Q., Hunter, L., Li, M., Marin-Padilla, M., Prakash, Y., Somlo, S., Harris, P., Torres, V., and Sieck, G.: Pkd2 Haploinsufficiency Alters Intracellular Calcium Regulation in Vascular Smooth Muscle Cells, Hum Mol Genet 12(15), 1875, 2003 |
| Elastase: .005% | |||||
| Collagenase: 0.05% | |||||
| Deoxyribonuclease I: 1000 u/ml | |||||
| PDS kit: with modifications | |||||
| 小鼠 | 小鼠 | Cardiomyocytes | Collagenase Type 2: 0.2% | DMEM/F-12 | Rybkin, I., Markham, D., Yan, Z., Bassel-Duby, R., Williams, R., and Olson, E.: Conditional Expression of SV40 T-antigen in Mouse Cardiomyocytes Facilitates an Inducible Switch from Proliferation to Differentiation, J Biol Chem 278(18), 15927, 2003 |
| Pancreatin: 0.06% | |||||
| 小鼠 | 小鼠, neonatal 2-4 day | Atrial myocytes | NCIS kit: with modifications see reference | L-15 | Bettahi, I,, Marker, C., Roman, M., and Wickman, K.: Contribution of the Kir3.1 Subunit to the Muscarinic-gated Atrial Potassium Channel IKACh, J Biol Chem 277, 48282, 2002 |
| 小鼠 | 小鼠, neonatal and adult | Cardiomyocytes | Collagenase Type 2: 0.03-0.2% | Joklik's MEM | Schreiber, K., Paquet, L., Allen, B., and Rindt, H.: Protein Kinase C Isoform Expression and Activity in the Mouse Heart, Am J Physiol Heart Circ Physiol281(5), H2062, 2001 |
| 小鼠 | 小鼠, NTg and Tg, 8 - 12 week | Myocytes | Collagenase Type 2: 150 u/ml | Joklik's MEM | Nelson, D., Setser, E., Hall, D., Schwartz, S., Hewitt, T., Klevitsky, R., Osinska, H., Bellgrau, D., Duke, R., and Robbins, J.: Proinflammatory Consequences of Transgenic Fas Ligand Expression in the Heart, J Clin Invest 105 (9), 1199, 2000 |
| 小鼠 | 小鼠, 1-6 day | Cardiomyocytes | Collagenase Type 2: 0.1% | PBS | Watzka SB, Lucien J, Shimada M, Edwards V, Yeger H, Hannigan G, and Coles JG.: Selection of viable cardiomyocytes for cell transplantation using three-dimensional tissue culture, Transplantation 70, 1310, 2000 |
| Deoxyribonuclease I: 0.002% | |||||
| 小鼠 | 小鼠, 2-4 mo | Ventricular myocytes | Collagenase Type 2: 0.05% | MEM | Zhou YY, Wang SQ, Zhu WZ, Chruscinski A, Kobilka BK, Ziman B, Wang S, Lakatta EG, Cheng H, Xiao RP: Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology, Am J Physiol Heart Circ Physiol 279, H429-36, 2000 |
| Collagenase Type 4: 0.05% | |||||
| Protease XIV: 0.002% | |||||
| 小鼠 | 小鼠 | Cardiomyocytes | Collagenase Type 2: 150 u/ml | Joklik's MEM | Christensen G, Minamisawa S, Gruber PJ, Wang Y, Chien KR: High-efficiency, long-term cardiac expression of foreign genes in living mouse embryos and neonates, Circulation 101, 178-84, 2000 |
| 小鼠 | 小鼠, 1-3 day | Cardiomyocytes | Trypsin: 0.25% | FBS-MEM | Wang, G., and Kang, Y.: Inhibition of Doxorubicin Toxicity in Cultured Neonatal Mouse Cardiomyocytes with Elevated Metallothionein Levels, J Pharmacol Exp Ther 288(3), 938, 1999 |
| 小鼠 | 小鼠, 1 day | Cardiomyocytes | NCIS kit: with modifications see reference | L-15 | Lader, A., Kwiatkowski, D., and Cantiello, H.: Role of Gelsolin in the Actin Filament Regulation of Cardiac L-type Calcium Channels, Am J Physiol 277, C1277, 1999 |
| 小鼠 | 小鼠, 3-7 month | Fibroblasts, mesangial, smooth muscle | Trypsin: 0.25% | DMEM | Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH: Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation, Mol Biol Cell 10, 1569-79, 1999 |
| Collagenase: see reference | |||||
| Soybean Trypsin Inhibitor: .05% | |||||
| 小鼠 | 小鼠 (BALB/c), adult | Myocytes | Collagenase Type 2: 0.04% | Tyrode's solution | Felzen, B., Shilkrut, M., Less, H., Sarapov, I., Maor, G., Coleman, R., Robinson, R., et al.: Fas (CD95/Apo-1)-Mediated Damage to Ventricular Myocytes Induced by Cytotoxic T Lymphocytes from Perforin-Deficient Mice,Circ Res 82, 438, 1998 |
| 小鼠 | 小鼠, 1 day | Cardiomyocytes | NCIS kit: with modifications see reference | L-15 | Lader, A., Xiao, Y., Ishikawa, Y., Cui, Y., Vatner, D., Vatner, S., Homcy, C., Cantiello, H.: Cardiac Gsalpha Overexpression Enhances L-type Calcium Channels Through an Adenylyl Cyclase Independent Pathway, Proc Natl Acad Sci U S A 95, 9669, 1998 |
| 小鼠 | 小鼠, P0-P1 | Cardiomyocytes | Collagenase: 0.045% | DMEM, Medium 199 | Arber, S., Hunter, J., Ross, J., Hongo, M., Sansig, G., Borg, J., Perriard, J., Chien, K., and Caroni, P.: MLP-Deficient Mice Exhibit a Disruption of Cardiac Cytoarchitectural Organization, Dilated Cadiomyopathy, and Heart Failure,Cell 88, 393, 1997 |
| 小鼠 | 小鼠, 6-9 or 21-41 day | Ventricular and atrial myocytes | Collagenase Type 2: 95 u/ml | DMEM/Tyrodes | Valenzuela, D., Han, X., Mende, U., Fankhauser, C., Mashimo, H., Huang, P., Pfeffer, J., Neer, E., and Fishman, M.: G alpha(o) is Necessary for Muscarinic Regulation of Ca2+ Channels in Mouse Heart, Proc Natl Acad Sci U S A 94(5), 1727, 1997 |
| Hyaluronidase: 172.5 u/ml | |||||
| Trypsin: 0.002% | |||||
| Deoxyribonuclease I: 60u/ml | |||||
| 小鼠 | 小鼠 | Cardiomyocyte | Collagenase: 0.17 % | PBS | Soonpaa, M., Kim, K., Pajak, aL., Franklin, M., and Field, L.:Cardiomyocyte DNA Synthesis and Binucleation During Murine Development,Am J Physiol 271, H2183, 1996 |
| 小鼠 | 小鼠 embryos | Myocytes | Collagenase Type 1: 0.1% | DMEM | Wobus, A., Kleppisch, T., Maltsev, V., and Hescheler, J.: Cardiomyocyte-Like Cells Differentiated In Vitro From Embryonic Carcinoma Cells P19 are Characterized by Functional Expression of Adrenoceptors and Ca2+ Channels,In Vitro Cell Dev Biol 30A, 425, 1994 |
| 小鼠 | 小鼠 | Myocytes | Trypsin NF 1:250: 0.25% | Rinaldini's buffer solution, CF | Gross, W., Schopf-Ebner, E., and Bucher, O.: Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells, Exp Cell Res 53, 1, 1968 |
| (also rat, neonate and chick, 8 day embryo) | |||||
| 綿羊 | 綿羊 | Atrial myocytes | Collagenase Type 2: 0.055% | Tyrode's solution | Voigt, N., Pearman, C., Dobrev, D. and Dibb, K.: Methods for Isolating Atrial Cells from Large Mammals and Humans., J Mol Cell Cardiol 86, 187-98, 2015 |
| Protease XIV: 0.006% | |||||
| 綿羊 | 綿羊 | Cardiomyocytes | Collagenase Type 2: 0.055% | See reference | Dibb, K., Clarke, J., Horn, M., Richards, M., Graham, H., Eisner, D. and Trafford, A.: Characterization of an Extensive Transverse Tubular Network in Sheep Atrial Myocytes and its Depletion in Heart Failure., Circ Heart Fail 2, 482-9, 2009 |
| Protease Type XIV: 0.006% | |||||
| 綿羊 | 綿羊 | Cardiomyocytes | Collagenase Type 2: 100-200 u/ml | See reference | Dhamoon, A, Pandit, S., Sarmast, F., Parisian, K., Guha, P., Li, Y., Bagwe, S., Taffet, S. and Anumonwo, J.: Unique Kir2.x Properties Determine Regional and Species Differences in the Cardiac Inward Rectifier K+ Current.,Circ Res 94, 1332-9, 2004 |
| 豬 | 豬 | Aortic smooth muscle and intersitial cells | Collagenase: 600 u/ml | PBS | Butcher, J. and Nerem, R.: Porcine Aortic Valve Interstitial Cells in Three-Dimensional Culture: Comparison of Phenotype with Aortic Smooth Muscle Cells., J Heart Valve Dis 13, 478, 2004 |
| 豬 | 豬 | Coronary vascular smooth muscle (PCVSMCs) | Trypsin: 0.037% | HEPES | Christ, M., Gunther, A., Heck, M., Schmidt, B., Falkenstein, E., and Wehling, M.: Aldosterone, Not Estradiol, Is the Physiological Agonist for Rapid Increases in cAMP in Vascular Smooth Muscle Cells, Circulation 99, 1485, 1999 |
| 豬 | Miniature swine, adult, female, 25-40 kg, familiarized with treadmill exercise | Coronary smooth muscle | Trypsin: 0.1% | Krebs bicarbonate solution | Bowles, D., Hu, Q., Laughlin, M., and Sturek, M.: Exercise Training Increases L-type Calcium Current Density in Coronary Smooth Muscle, Am J Physiol 275 (44), H2159, 1998 |
| 兔 | 兔, New Zealand, 2.3-2.9 kg | Cardiomyocytes | Collagenase Type 1: 0.05% | Krebs-Henseleit | Farkas, A., Acsai, K., Nagy, N., Toth, A., Fulop, F., Seprenyi, G., Birinyi, P., Nanasi, P., Forster, T., Csanady, M., Papp, J., Varrro, A. and Farkas, A.:Na(+)/Ca(2+) Exchanger Inhibition Exerts a Positive Inotropic Effect in the Rat Heart, but Fails to Influence the Contractility of the Rabbit Heart., Br J Pharmacol 154, 93, 2008 |
| Hyaluronidase: see reference | |||||
| Protease: see reference | |||||
| 兔 | 兔 | Smooth muscle cells | Collagenase Type 2: 300 u/ml | F10 Ham | Verheye, S., Martinet, W., Kockx, M., Knaapen, M., Salu, K., Timmermans, J., Ellis, J., Kilpatrick, D., DeMayer, G.: Selective Clearance of Macrophages in Atherosclerotic Plaques by Autophagy, J Am Coll Cardiol49, 706, 2007 |
| Elastase: 5 u/ml | |||||
| 兔 | 兔, New Zealand, 2 kg | Cardiomyocytes and fibroblasts | Collagenase: 0.06% | Medium 199 | Driesen Ronald B, Dispersyn Gerrit D, Verheyen Fons K, van den Eijnde Stefan M, Hofstra Leo, Thone Fred, Dijkstra Petra, Debie Wiel, Borgers Marcel, Ramaekers Frans C S: Partial cell fusion: a newly recognized type of communication between dedifferentiating cardiomyocytes and fibroblasts,Cardiovasc Res 68, 37-46, 2005 |
| 兔 | 兔, New Zealand White, 2-3 kg | Myocytes | Collagenase Type 2: 0.1% | HEPES | Spitzer, K., Ershler, P., Skolnick, R., and Vaughan-Jones, R.: Generation of Intracellular pH Gradients in Single Cardiac Myocytes with a Microperfusion System, Am J Physiol 278, H1371, 2000 |
| 兔 | 兔, New Zealand, white, either sex | Myocytes | Collagenase Type 2: 0.05% | EGTA-KB | Sedarat, F., Xu, L., Moore, E., and Tibbits, G.: Colocalization of Dihydropyridine and Ryanodine Receptors in Neonate Rabbit Heart Using Confocal Microscopy, Am J Physiol Heart Circ Physiol 279, H202, 2000 |
| 兔 | 兔, adult 1.5-2.5 kg | Tricuspid valve cells | Collagenase: 0.8 mg/ml | HEPES Tyrode solution | Anumonwo JM, Delmar M, and Jalife J.: Electrophysiology of single heart cells from the rabbit tricuspid valve, J Physiol 425, 145, 1990 |
| 兔 | 兔, New Zealand, white, male, 2-3 kg | Cardiomyocytes | Hyaluronidase: 0.5% | Eagle's MEM | Buxton, I., and Brunton, L.: Compartments of Cyclic AMP and Protein Kinase in Mammalian Cardiomyocytes, J Biol Chem 258 (17), 10233, 1983 |
| 兔 | 兔, adult, 2 kg | Myocytes | Hyaluronidase: 0.007% | Kreb's Ringer | Dani, A., Cittadini, A., Flamini, G., Festuccia, G., and Terranova, T.:Preparation and Some Properties of Isolated Beating Myocytes from Adult Rabbit Heart, J Mol Cell Cardiol 9, 777, 1977 |
| 大鼠 | 大鼠, 1-2 day | Cardiomyocytes | NCIS kit: per instructions | MEM | Xie, L., Pi, X., Townley-Tilson, WH., Li, N., Wehrens, X., Entman, M., Taffet, G., Mishra, A., Peng, J., Schisler, J., Meissner, G. and Patterson, C.: PHD2/3-dependent Hydroxylation Tunes Cardiac Response to 尾-adrenergic Stress via Phospholamban., J Clin Invest 125, 2759-71, 2015 |
| 大鼠 | 大鼠, Wistar, 250-350g | Ventricular myocytes | Collagenase Type 2: 251 u/ml | Tyrode solution | Calderon-Sachez, E., Domi璶guez-Rodriguez, A., Lopez-Haldon, J., Jimenez-Navarro, M., Gomez, A., Smani, T. and Ordonez, A.:Cardioprotective Effect of Ranolazine in the Process of Ischemia-reperfusion in Adult Rat Cardiomyocytes., Rev Esp Cardiol (Engl Ed) , , 2015 |
| 大鼠 | 大鼠, SD, 8-10 week | Ventricular myocytes | Collagenase: 0.08% | Tyrode's Solution | Lee, J., Ha, J. and Leem, C.: A Novel Nicotinamide Adenine Dinucleotide Correction Method for Mitochondrial Ca(2+) Measurement with FURA-2-FF in Single Permeabilized Ventricular Myocytes of Rat., Korean J Physiol Pharmacol 19, 373-82, 2015 |
| Pronase: 0.004% | |||||
| 大鼠 | 大鼠, SD, 1 day | Cardiomyocytes | Trypsin: 0.025% | HBSS | Clark, A. and Naya, F.: MicroRNAs in the Myocyte Enhancer Factor 2 (MEF2)-regulated Gtl2-Dio3 Noncoding RNA Locus Promote Cardiomyocyte Proliferation by Targeting the Transcriptional Coactivator Cited2., J Biol Chem 290, 23162-72, 2015 |
| Collagenase Type 2: 1.0% | |||||
| 大鼠 | 大鼠, SD, 2-3 day | Cardiomyocytes | Collagenase Type 2: 0.05% | DMEM | Fang, R, Qiao, S., Liu, Y., Meng, Q., Chen, X., Song, B., Hou, X. and Tian, W.: Sustained Co-Delivery of BIO and IGF-1 by a Novel Hybrid Hydrogel System to Stimulate Endogenous Cardiac Repair in Myocardial Infarcted Rat Hearts., Int J Nanomedicine 10, 4691-703, 2015 |
| 大鼠 | 大鼠, SD | Valve Interstitial cells | Collagenase Type 2: 600 u/ml | DMEM | Horne, T., VandeKopple, M., Sauls, K., Koenig, S., Anstine, L., Garg, V., Norris, R. and Lincoln, J.: Dynamic Heterogeneity of the Heart Valve Interstitial Cell Population in Mitral Valve Health and Disease, J Cardiovasc Dev Dis 2, 214, 2015 |
| 大鼠 | Wistar, 200 g | Ventricular myocytes | Collagenase Type 2: 95 u/ml | HEPES | Miller, L., Greensmith, D., Sankaranarayanan, R., O'Neill, S. and Eisner D.:The Effect of 2,5-di-(tert-butyl)-1,4-benzohydroquinone (TBQ) on Intracellular Ca2+ Handling in Rat Ventricular Myocytes., Cell Calcium 58, 208-14, 2015 |
| Protease: 0.1 u/ml | |||||
| 大鼠 | 大鼠, SD, 3-4 month | Cardiomyocytes | Collagenase Type 2: 0.1% | DMEM | Liu, L., Wang, Y., Cao, Z., Wang, M., Liu, X., Gao, T., Hu, Q., Yuan, W. and Lin, L.: Up-regulated TLR4 in Cardiomyocytes Exacerbates Heart Failure after Long-Term Myocardial Infarction., J Cell Mol Med 19, 2728, 2015 |
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | NCIS kit: per instructions | HBSS | Song, D., Lee, K., Ryu, J., Jeon, H. and Kim, D.: The Molecular Interaction of Heart LIM Protein (HLP) with RyR2 and Caveolin-3 is Essential for Ca(2+)-Induced Ca(2+) Release in the Heart., Biochem Biophys Res Commun 463, 975-81, 2015 |
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | Trypsin: 0.06% | HBSS | Zhang, B., Montgomery, M., Davenport-Huyer, L., Korolj, A. and Radisic, M.: Platform Technology for Scalable Assembly of Instantaneously Functional Mosaic Tissues., Sci. Adv. 1, e1500423, 2015 |
| Collagenase Type 2: 220 u/ml | |||||
| 大鼠 | 大鼠 | Cardiomyocytes | Collagenase Type 2: 150 u/ml | MEM | Hennessey, J., Wei, E. and Pitt, G.: Fibroblast Growth Factor Homologous Factors Modulate Cardiac Calcium Channels., Circ Res 113, 381-8, 2013 |
| 大鼠 | 大鼠, Wistar, 175-200g | Cardiomyocytes | Collagenase Type 2: 150 u/ml | see reference | Eckerle, L., Felix, S. and Herda, L.: Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes., J Vis Exp 73, e4237, 2013 |
| 大鼠 | 大鼠, SD, 1-2 day | Cardiomyocytes | Trypsin: 0.06% | DMEM | Zhang, B., Green, J., Murthy, S. and Radisic, M.: Label-Free Enrichment of Functional Cardiomyocytes using Microfluidic Deterministic Lateral Flow Displacement., PLoS ONE 7, e37619, 2012 |
| Collagenase Type 2: 220 u/ml | |||||
| 大鼠 | 大鼠, adult | Ventricular myocytes | Collagenase Type 2: 0.2% | Medium 199 | Karakikes, I., Hadri, L., Rapti, K., Ladage, D., Ishikawa, K., Tilemann, L., Yi, G., Morel, C., Gwathmey, J., Zsebo, K., Weber, T., Kawase, Y. and Hajjar, R.: Concomitant Intravenous Nitroglycerin with Intracoronary Delivery of AAV1.SERCA2a Enhances Gene Transfer in Porcine Hearts., Mol Therapy 20, 565, 2012 |
| 大鼠 | 大鼠, 3 day | Cardiomyocytes | Collagenase Type 2: 300 u/ml | PBS | Ye, K., Sullivan, K. and Black, L.: Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering., J Vis Exp 55, 3251, 2011 |
| 大鼠 | 大鼠, SD, male | Cardiomyocytes | Collagenase Type 2: 0.056% | Krebs | Mellor, K., Bell, J., Wendt, I., Davidoff, A., Ritchie, R. and Delbridge, L.:Fructose Modulates Cardiomyocyte Excitation-Contraction Coupling and Ca Handling In Vitro., PLoS ONE 6, e25204, 2011 |
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | NCIS kit: per instructions | L-15 | Dittami, G., Rajguru, S., Lasher, R., Hitchcock, R. and Rabbitt, R.:Intracellular Calcium Transients Evoked by Pulsed Infrared Radiation in Neonatal Cardiomyocytes., J Physiol 589, 1295-306, 2011 |
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | Trypsin: 0.07% | DMEM/M199 | Guan, X., Delo, D., Atala, A. and Soker, S.: In Vitro Cardiomyogenic Potential of Human Amniotic Fluid Stem Cells., J Tissue Eng Regen Med 5, 220, 2011 |
| Collagenase Type 2: 0.1% | |||||
| 大鼠 | 大鼠, adult | Cardiomyocytes | Collagenase Type 2: 0.025% | see reference | Louch, W., Sheehan, K. and Wolska, B.: Methods in Cardiomyocyte Isolation, Culture, and Gene Transfer., J Mol Cell Cardiol 51, 288-98, 2011 |
| 大鼠 | 大鼠, Wistar, male | Cardiomyocytes | NCIS kit: per instructions | L-15 | Brinckmann, M., Kaschina, E., Altarche-Xifro, W., Curato, C., Timm, M., Grzesiak, A., Dong, J., Kappert, K., Kintscher, U., Unger, T. and Li, J.:Estrogen Receptor Alpha Supports Cardiomyocytes Indirectly Through Post-Infarct Cardiac c-kit+ Cells., J Mol Cell Cardiol 47, 66, 2009 |
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | NCIS kit: per instructions | L-15 | Smith, M., Huang, Y. and Deshmukh, M.: Skeletal Muscle Differentiation Evokes Endogenous XIAP to Restrict the Apoptotic Pathway., PLoS ONE 4, e5097, 2009 |
| 大鼠 | 大鼠, adult | Myocytes | Collagenase Type 2: 0.05% | Media 199 | Xu, X. and Colecraft, H.: Primary Culture of Adult Rat Heart Myocytes., J Vis Exp 28, 1308, 2009 |
| Protease XIV: 0.02% | |||||
| 大鼠 | 大鼠, SD, 200-250 g | Cardiomyocytes | Collagenase Type 1: 0.05% | Krebs-Henseleit | Farkas, A., Acsai, K., Nagy, N., Toth, A., Fulop, F., Seprenyi, G., Birinyi, P., Nanasi, P., Forster, T., Csanady, M., Papp, J., Varrro, A. and Farkas, A.:Na(+)/Ca(2+) Exchanger Inhibition Exerts a Positive Inotropic Effect in the Rat Heart, but Fails to Influence the Contractility of the Rabbit Heart., Br J Pharmacol 154, 93, 2008 |
| Hyaluronidase: see reference | |||||
| Protease: see reference | |||||
| 大鼠 | 大鼠, SD, 1-2 day | Cardiomyocytes | NCIS kit: per instructions | L-15 | Kim, M., Oh, J., Sakata, S., Liang, I., Park, W., Hajjar, R. and Lebeche, D.:Role of Resistin in Cardiac Contractility and Hypertrophy., J Mol Cell Cardiol 45, 270, 2008 |
| 大鼠 | 大鼠, SD, 2 day | Cardiomyocytes | Collagenase Type 2: 0.1% | DMEM/F-12 | Jang, J., Ku, S., Kim, J., Choi, K., Kim, Y., Kim, H., Oh, S., Lee, E., Cho, H., Song, Y., Lee, S., Lee, S., Suh, C., Kim, S., Moon, S. and Choi, Y.: Notch Inhibition Promotes Human Embryonic Stem Cell-Derived Cardiac Mesoderm Differentiation., Stem Cells 26, 2782, 2008 |
| 大鼠 | 大鼠, SD, male, 225-250g | Cardiomyocytes | Collagenase Type 2: 0.1% | J-MEM | Kubli, D., Quinsay, M., Huang, C., Lee, Y., Gustafsson, A.: Bnip3 Functions as a Mitochondrial Sensor of Oxidative Stress During Myocardial Ischemia and Reperfusion., Am J Physiol Heart Circ Physiol 295, H2025, 2008 |
| 大鼠 | 大鼠, 2-3 day | Cardiomyocytes | Collagenase Type 2: 0.05% | DMEM | LaFramboise, W., Scalise, D., Stoodley, P., Graner, S., Guthrie, R., Magovern, J and Becich, M.: Cardiac Fibroblasts Influence Cardiomyocyte Phenotype in Vitro., Am J Physiol Cell Physiol 292, C1799-808, 2007 |
| Pancreatin: 0.1% | |||||
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | NCIS kit: per instructions | L-15 | Robinet, A., Millart, H., Oszust, F., Hornebeck, W. and Bellon, G.: Binding of Elastin Peptides to S-Gal Protects the Heart Against Ischemia/Reperfusion Injury by Triggering the RISK Pathway., FASEB J 21, 1968, 2007 |
| 大鼠 | 大鼠, SD, 300-350 g | Cardiomyocytes | Collagenase Type 2: 0.05% | DMEM | Niederbichler Andreas D, Hoesel Laszlo M, Westfall Margaret V, Gao Hongwei, Ipaktchi Kyros R, Sun Lei, Zetoune Firas S, Su Grace L, Arbabi Saman, Sarma J Vidya, Wang StewartC, Hemmila MarkR, Ward PeterA:An essential role for complement C in the pathogenesis of septic cardiac dysfunction, J Exp Med 203, 53-61, 2006 |
| Hyaluronidase: 0.02% | |||||
| 大鼠 | 大鼠, SD, male | Ventricular myocytes | Collagenase: 0.13% | DMEM | Stagg Mark A, Coppen Steven R, Suzuki Ken, Varela-Carver Anabel, Lee Joon, Brand Nigel J, Fukushima Satsuki, Yacoub Magdi H, Terracciano Cesare M: Evaluation of frequency, type, and function of gap junctions between skeletal myoblasts overexpressing connexin43 and cardiomyocytes: relevance to cell transplantation, FASEB J 20, 744-6, 2006 |
| Hyaluronidase: 0.06% | |||||
| 大鼠 | 大鼠, SD, 200-250 g | Ventricular myocytes and mesenteric arterial SMC | Collagenase Type 2: 0.06% | Krebs-Ringer | Lu Tong, Ye Dan, Wang Xiaoli, Seubert John M, Graves Joan P, Bradbury J Alyce, Zeldin Darryl C, Lee Hon-Chi: Cardiac and vascular KATP channels in rats are activated by endogenous epoxyeicosatrienoic acids through different mechanisms, J Physiol 575, 627-44, 2006 |
| Papain: 0.175% | |||||
| 大鼠 | 大鼠, Wistar, neonatal | Cardiomyocytes | NCIS kit: per instructions | L-15 | Butler, T., Au, C., Yang, B., Egan, J., Tan, Y., Hardeman, E., North, K., Verkman, A. and Winlaw, D.: Cardiac Aquaporin Expression in Humans, Rats, and Mice., Am J Physiol Heart Circ Physiol 291, H705, 2006 |
| 大鼠 | 大鼠, 1-2 day | Cardiomyocytes | NCIS kit: per instructions | DMEM/F-12 | Pedram, A., Razandi, M., Aitkenhead, M., and Levin, E: Estrogen Inhibits Cardiomyocyte Hypertrophy in vitro. Antagonism of Calcineurin-related Hypertrophy through Induction of MCIP1, J Biol Chem 280, 26339, 2005 |
| 大鼠 | 大鼠, SD, male, 250-300 g | Cardiomyocytes | Collagenase Type 2: 250 u/ml | Cardioplegic solution | Head, B., Pa, H., Roth, D., Lai, N., Niesman, I., Farquhar, M. and Insel, P.: G-Protein-Coupled Receptor Signaling Components Localize in Both Sarcolemmal and Intracellular Caveolin-3-Associated Microdomains in Adult Cardiac Myocytes., J Biol Chem 280, 31036-44, 2005 |
| 大鼠 | 大鼠, Wistar, 1-3 day | Cardiomyocytes | NCIS kit: per instructions | L-15 | Castillo, A., Ruzmetov, N., Harvey, K., Stillwell, W., Zaloga, G. and Siddiqui, R.: Docosahexaenoic Acid Inhibits Protein Kinase C Translocation/Activation and Cardiac Hypertrophy in Rat Cardiomyocytes., J Mol Genet Med 1, 18, 2005 |
| 大鼠 | 大鼠, SD, 2-4 day | Cardiomyocytes, fibroblasts | Trypsin: 0.1% | Medium 199 | Entcheva, E., Bien, H, Yin L., Chung, C., Farrell, M., and Kostov, Y.:Functional Cardiac Cell Constructs on Cellulose-based Scaffolding,Biomaterials 25(26), 5753, 2004 |
| Collagenase: 0.1% | |||||
| 大鼠 | 大鼠, SD, 1-2 day | Cardiomyocytes | NCIS kit: per instructions | L-15 | Muller-Bore, B., Cascio, W., Anderson, P., Snowwaert, J., Frye, J., Desai, N., Esch, G., Brackham, J., Bagnell, C., Coleman, W., Grisham, J., and Malouf, N.: Adult-derived Liver Stem Cells Acquire a Cardiomyocyte Structural and Functional Phenotype ex vivo, Am J Pathol 165, 135, 2004 |
| 大鼠 | 大鼠,1-2 day | Cardiomyocytes | NCIS kit: per instructions | DMEM | Natarajan AR, Rong Q, Katchman AN, and Ebert SN.: Intrinsic cardiac catecholamines help maintain beating activity in neonatal rat cardiomyocyte cultures, Pediatr Res 56, 411, 2004 |
| 大鼠 | 大鼠, 1 day | Cardiomyocytes | NCIS kit: per instructions | F-10 | Chen, Z., Ge, Y., and Kang J.: Down-regulation of the M6P/IGF-II Receptor Increases Cell Proliferation and Reduces Apoptosis in Neonatal Rat Cardiac Myocytes, BMC Cell Biol 5, 15, 2004 |
| 大鼠 | 大鼠, neonatal | Cardiac myocytes | Collagenase Type 2: 0.5% | HBSS | Chen Hsiao-Huei, Mullett Steven J, Stewart Alexandre F R: Vgl-4, a novel member of the vestigial-like family of transcription cofactors, regulates alpha1-adrenergic activation of gene expression in cardiac myocytes, J Biol Chem 279, 30800-6, 2004 |
| 大鼠 | 大鼠, male, 250-300 g | Cardiomyocytes | Collagenase Type 1: 0.03% | HBSS | Hunton DaciaL, Zou LuYun, Pang Yi, Marchase Richard B: Adult rat cardiomyocytes exhibit capacitative calcium entry, Am J Physiol Heart Circ Physiol 286, H1124-32, 2004 |
| Protease: 0.01% | |||||
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | Collagenase Type 2: 0.1% | DMEM | Tamamori-Adachi, M., Hayashida, K., Nobori, K., Omizu, C., Yamada, K., Sakamoto, N., Kamura, T., Fukuda, K., Ogawa, S., Nakayama, K. and Kitajima, S.: Down-Regulation of p27 Promotes Cell Proliferation of Rat Neonatal Cardiomyocytes Induced by Nuclear Expression of Cyclin D1 and CDK4, J Biol Chem 2279, 50429, 2004 |
| 大鼠 | 大鼠, fetal or 3-day | Cardiomyocytes | Collagenase Type 2: 0.1% | HEPES | Tamamori-Adachi Mimi, Ito Hiroshi, Sumrejkanchanakij Piyamas, Adachi Susumu, Hiroe Michiaki, Shimizu Masato, Kawauchi Junya, Sunamori Makoto, Marumo Fumiaki, Kitajima Shigetaka, Ikeda Masa-Aki: Critical role of cyclin D1 nuclear import in cardiomyocyte proliferation, Circ Res 92, e12-9, 2003 |
| 大鼠 | 大鼠, adult, 220-280g | Cardiomyocytes | Collagenase Type 2: 178 u/ml | Krebs-Henseleit, CF | Gordon Jennifer M, Dusting Gregory J, Woodman Owen L, Ritchie Rebecca H: Cardioprotective action of CRF peptide urocortin against simulated ischemia in adult rat cardiomyocytes, Am J Physiol Heart Circ Physiol 284, H330-6, 2003 |
| Hyaluronidase: 0.01% | |||||
| 大鼠 | 大鼠, 50 g | Ventricular myocytes | Collagenase: 223 u/ml | Medium 199 | Aberle II NS, and Ren J.: Experimental Assessment of the Role of Acetaldehyde in Alcoholic Cardiomyopathy, Biol Proced Online 5, 1, 2003 |
| Hyaluronidase: 0.01% | |||||
| Trypsin: 0.002% | |||||
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | NCIS kit: per instructions | L-15 | Li TS, Ito H, Kajiwara K, and Hamano K.: Long-term survival of xenografted neonatal cardiomyocytes by adenovirus-mediated CTLA4-Ig expression and CD40 blockade, Circulation 108, 1760, 2003 |
| 大鼠 | 大鼠, SD, 200-300 g | Cardiomyocytes | Collagenase Type 2: 0.05-0.1% | Joklik's MEM | Swift Luther, McHowat Jane, Sarvazyan Narine: Inhibition of membrane-associated calcium-independent phospholipase A2 as a potential culprit of anthracycline cardiotoxicity, Cancer Res 63, 5992-8, 2003 |
| 大鼠 | 大鼠, SD, 175-200g | Cardiomyocytes | Collagenase Type 2: 0.05% | Krebs-Ringer | Tardif Annie, Julien Nathalie, Chiasson Jean-Louis, Coderre Lise:Stimulation of glucose uptake by chronic vanadate pretreatment in cardiomyocytes requires PI 3-kinase and p38 MAPK activation, Am J Physiol/Endo 284, E1055-64, 2003 |
| Deoxyribonuclease I: 0.02% | |||||
| 大鼠 | 大鼠, SD, pregnant female | Peritoneal mast | Hyaluronidase: 100 u/ml | DMEM (see reference) | DeAlmeida, A., Mustin, D., Forman, M., Brower, G., Janicki, J., and Carver, W.: Effects of mast cells on the behavior of isolated heart fibroblasts: modulation of collagen remodeling and gene expression, J Cell Physiol 191, 51, 2002 |
| 大鼠 | 大鼠, neonatal, adult, 17 day | Ventricular cardiomyocytes | Collagenase Type 2: 0.7-1% | DMEM | Lam ML, Bartoli M, and Claycomb WC.: The 21-day postnatal rat ventricular cardiac muscle cell in culture as an experimental model to study adult cardiomyocyte gene expression, Mol Cell Biochem 229, 51, 2002 |
| 大鼠 | 大鼠, 1 day | Cardiomyocytes | NCIS kit: per instructions | L-15 | Yang Y, Liao H, Ke Q, Cai J, Xiao YF, and Morgan JP: Enhancement of nitric oxide production by methylecgonidine in cultured neonatal rat cardiomyocytes, Br J Pharmacol 135, 188, 2002 |
| 大鼠 | 大鼠, female, 250-300 g | Ventricular myocytes | Collagenase Type 2: 0.1% | Ringer solution | Liu Qinghang, Hofmann Polly A: Antiadrenergic effects of adenosine A(1) receptor-mediated protein phosphatase 2a activation in the heart, Am J Physiol Heart Circ Physiol 283, H1314-21, 2002 |
| 大鼠 | 大鼠, Wistar, 1 day | Cardiomyocytes | Collagenase Type 2: 80u/ml | M199 | Shimizu Tatsuya, Yamato Masayuki, Isoi Yuki, Akutsu Takumitsu, Setomaru Takeshi, Abe Kazuhiko, Kikuchi Akihiko, Umezu Mitsuo, Okano Teruo: Fabrication of pulsatile cardiac tissue grafts using a novel 3-dimensional cell sheet manipulation technique and temperature-responsive cell culture surfaces, Circ Res 90, e40, 2002 |
| 大鼠 | 大鼠, male, 6-12 months old | Cardiac myocytes | Protease: 0.55 u/ml | Tyrode's solution | Kitta, K., Day, R., Ikeda, T., and Suzuki, Y.: Hepatocyte Growth Factor Protects Cardiac Myocytes Against Oxidative Stress-Induced Apoptosis, Free Radic Biol Med 31 (7), 902, 2001 |
| 大鼠 | 大鼠, Harlan SD, pup | Cardiomyocytes | Trypsin: | DMEM/Ham's F-12 | Schwartzbauer, G., and Robbins, J.: The Tumor Suppressor Gene PTEN Can Regulate Cardiac Hypertrophy and Survival, J Biol Chem 276, 35786, 2001 |
| 大鼠 | 大鼠, SD, newborn | Cardiac myocytes | NCIS kit: per instructions | L-15 | Rahman A, Alam M, Rao S, Cai L, Clark LT, Shafiq S, and Siddiqui MA:Differential effects of doxorubicin on atrial natriuretic peptide expression in vivo and in vitro, Biol Res 34(3-4), 195, 2001 |
| 大鼠 | 大鼠, adult | Ventricular myocytes | Collagenase: 223 u/ml | Tyrode solution | Ren J, and Wold LE.: Measurement of Cardiac Mechanical Function in Isolated Ventricular Myocytes from Rats and Mice by Computerized Video-Based Imaging, Biol Proced Online 3, 43, 2001 |
| Hyaluronidase: 0.01% | |||||
| Trypsin: 0.002% | |||||
| 大鼠 | 大鼠, SD, male | Cardiomyocytes | Collagenase Type 2: 140 u/ml | Krebs-Henseleit | Dai L, Brookes PS, Darley-Usmar VM, and Anderson PG.: Bioenergetics in cardiac hypertrophy: mitochondrial respiration as a pathological target of NO, Am J Physiol/Heart 281, H2261-H2269, 2001 |
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | NCIS kit: per instructions | DMEM/M199 | Guo K, Searfoss G, Krolikowski D, Pagnoni M, Franks C, Clark K, Yu KT, Jaye M, Ivashchenko Y: Hypoxia induces the expression of the pro-apoptotic gene BNIP3, Cell Death Differ 8, 367-76, 2001 |
| 大鼠 | 大鼠, Wistar, female | Ventricular myocytes | Collagenase Type 2: 0.1% | Ringer solution | Pyle, W, Lester, J, and Hofmann, P.: Effects of Kappa-opioid receptor activation of myocardium, Am J Physiol Heart Circ Physiol 281, H669, 2001 |
| 大鼠 | 大鼠, SD, 1-2 day | Cardiomyocytes | Trypsin: 0.01% | HBSS | Arutunyan A, Webster D, Swift L, Sarvazyan N: Localized injury in cardiomyocyte network: a new experimental model of ischemia-reperfusion arrhythmias, Am J Physiol Heart Circ Physiol 280, H1905-15, 2001 |
| Collagenase Type 2: 0.08% | |||||
| 大鼠 | 大鼠, newborn | Atrial | Trypsin: 0.06% | DMEM | Kim, D., and Pleumsamran, A.: Cytoplasmic Unsaturated Free Fatty Acids Inhibit ATP-Dependent Gating of the G Protein-gated K+ Channel, J Gen Physiol 115, 287, 2000 |
| 大鼠 | 大鼠, 1-2 day | Cardiomyocytes | Collagenase Type 1: 84 u/ml | M199 medium | Bueno OF, De Windt LJ, Tymitz KM, Witt SA, Kimball TR, Klevitsky R, Hewett TE, Jones SP, Lefer DJ, Peng CF, Kitsis RN, and Molkentin JD.: The MEK1-ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice, EMBO J 19(23), 6341, 2000 |
| Pancreatin: 0.05% | |||||
| 大鼠 | 大鼠, Winstar, 1-2 day | Ventricular myocytes | Collagenase Type 2: 0.08% | DMEM | Maki T, Horio T, Yoshihara F, Suga S, Takeo S, Matsuo H, and Kangawa K: Effect of neutral endopeptidase inhibitor on endogenous atrial natriuretic peptide as a paracrine factor in cultured cardiac fibroblasts, Br J Pharmacol 131(6), 1204, 2000 |
| 大鼠 | 大鼠, SD, adult female | Heart microvascular cells | CLSPA: 250u/ml | L-15 | Puri S, Bansal DD, Uskokovic MR, and MacGregor RR.: Induction of tissue plasminogen activator secretion from rat heart microvascular cells by fM 1,25(OH)(2)D(3), Am J Physiol/Endo 278(2), E293, 2000 |
| Papain: 5 u/ml | |||||
| Elastase: 0.8 u/ml | |||||
| 大鼠 | 大鼠, SD, male, 250-300g | Cardiomyocytes | Collagenase Type 2: 0.06% | M199 | Sun L, Chang J, Kirchhoff SR, and Knowlton AA.: Activation of HSF and selective increase in heat-shock proteins by acute dexamethasone treatment, Am J Physiol Heart Circ Physiol 278(4), H1091, 2000 |
| 大鼠 | 大鼠, wistar, 2 day | Cardiomyocytes | NCIS kit: per instructions | DMEM-F12 | Cowan DB, Poutias DN, Del Nido PJ, and McGowan FX Jr: CD14-independent activation of cardiomyocyte signal transduction by bacterial endotoxin, Am J Physiol Heart Circ Physiol 279, H619, 2000 |
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | NCIS kit: per instructions | L-15 | Webster DR, and Patrick DL.: Beating rate of isolated neonatal cardiomyocytes is regulated by the stable microtubule subset, Am J Physiol Heart Circ Physiol 278, H1653, 2000 |
| 大鼠 | 大鼠, Lewis, adult male, 300-350-g | Aorta, smooth muscle | Trypsin: 0.025% | Medium 199 with 20% FBS medium | Kim, B., Nikolovski, J., Bonadio, J., Smiley, E., and Mooney, D.: Engineered Smooth Muscle Tissues: Regulating Cell Phenotype with the Scaffold, Exp Cell Res 251, 318, 1999 |
| 大鼠 | 大鼠, 10-15 newborn, 1-2 days old | Atrial | Trypsin: 0.03% | HBSS or EBSS | Nemec, J., Wickman, K., and Clapham, D.: GBy Binding Increases the Open Time of IKACh: Kinetic Evidence for Multiple GBy Binding Sites, Biophys J 76, 246, 1999 |
| 大鼠 | 大鼠, 1-3 day | Neonatal rat cardiomyocytes | Collagenase Type 2: 80u/ml | DMEM/F12 | Adderley SR, and Fitzgerald DJ.: Oxidative damage of cardiomyocytes is limited by extracellular regulated kinases 1/2-mediated induction of cyclooxygenase-2, J Biol Chem 274, 5038, 1999 |
| Pancreatin: 0.06% | |||||
| 大鼠 | 大鼠, 1-2 day | Cardiomyocytes | Pancreatin: 0.06% | DMEM | Reinecke H, Zhang M, Bartosek T, and Murry CE.: Survival, integration, and differentiation of cardiomyocyte grafts: a study in normal and injured rat hearts, Circulation 100(2), 193, 1999 |
| Collagenase Type 2: 95 u/ml | |||||
| 大鼠 | 大鼠, adult and neonatal | Cardioomyocytes | Collagenase Type 4: 0.12%-adult | DMEM/medium 199 | Richards SM, Jaconi ME, Vassort G, and Puceat M.: A spliced variant of AE1 gene encodes a truncated form of Band 3 in heart: the predominant anion exchanger in ventricular myocytes, J Cell Sci 112, 1519, 1999 |
| Collagenase Type 2: 0.05%-neonatal | |||||
| 大鼠 | 大鼠, Wistar, male | Cardiomyocytes | Collagenase Type 2: 228 u/ml | Joklik MEM | Sambandam N, Abrahani MA, St Pierre E, Al-Atar O, Cam MC, and Rodrigues B.: Localization of lipoprotein lipase in the diabetic heart: regulation by acute changes in insulin, Arterioscler Thromb Vasc Biol 19(6), 1526, 1999 |
| 大鼠 | 大鼠, 1-2 day | Cardiomyocytes | NCIS kit: per instructions | RPMI | Matsui, T., Li, Ling, Del Monte, F., Fukui, Y., Franke, T., Hajjar, R., Rosenzweig, A.: Adenoviral gene transfer of activated phosphatidylinositol 3'-kinase and Akt inhibits apoptosis of hypoxic cardiomyocytes in vitro,Circulation 100, 2373-9, 1999 |
| 大鼠 | 大鼠 | Thoracic aorta, smooth muscle | Elastase: 0.02% | (see reference) | Zilberman, A., Dave, V., Miano, J., Olson, E., and Periasamy, M.:Evolutionary Conserved Promoter Region Containing CArg-Like Elements is Crucial for Smooth Muscle Myosin Heavy Chain Gene Expression, Circ Res 82, 566, 1998 |
| 大鼠 | 大鼠, SD, 1 day | Cardiomyocytes | NCIS kit: per instructions | DMEM/F-12 | Wagner DR, Combes A, McTiernan C, Sanders VJ, Lemster B, Feldman AM: Adenosine inhibits lipopolysaccharide-induced cardiac expression of tumor necrosis factor-alpha, Circ Res 82, 47-56, 1998 |
| 大鼠 | 大鼠, SD, 300-350 g | Cardiomyocytes | Collagenase Type 2: 0.04% | see reference | Yu L, Netticadan T, Xu YJ, Panagia V, Dhalla NS: Mechanisms of lysophosphatidylcholine-induced increase in intracellular calcium in rat cardiomyocytes, J Pharmacol Exp Ther 286, 1-8, 1998 |
| 大鼠 | 大鼠, SD, female, 200g | Ventricular cardiac myocytes | Collagenase Type 2: 0.5% | Krebs-Henseleit buffer | Westfall M V, Rust E M, Metzger J M: Slow skeletal troponin I gene transfer, expression, and myofilament incorporation enhances adult cardiac myocyte contractile function, Proc Natl Acad Sci U S A 94, 5444-9, 1997 |
| Hyaluronidase: 0.2% | |||||
| 大鼠 | 大鼠, Wistar, 1 day | Cardiomyoctes | Collagenase Type 2: 80 u/ml | HBSS | Kinugawa, K., Kohmoto, O., Yao, A., Serizawa, T., and Takahashki, T.:Cardiac inducible nitric oxide synthase negatively modulates myocardial function in cultured rat myocytes, Am J Physiol 272, H35-47, 1997 |
| 大鼠 | 大鼠, neonatal, 5-day | Cardiomyocytes | Trypsin: 0.05% | DMEM | Vanwinkle, W., Snuggs, M., and Buja, L.: Cardiogel: A Biosynthetic Extracellular Matrix for Cardiomyocyte Culture, In Vitro Cell Dev Biol Anim 32, 478, 1996 |
| 大鼠 | 大鼠, SD, 250-300 g | Cardiomyocytes | Collagenase Type 2: 0.05% | Joklik's | Sharma VK, Colecraft HM, Wang DX, Levey AI, Grigorenko EV, Yeh HH, and Sheu SS: Molecular and functional identification of m1 muscarinic acetylcholine receptors in rat ventricular myocytes, Circ Res 79(1), 86, 1996 |
| 大鼠 | 大鼠, SD, fetal and neonatal | Cardiomyocytes | Collagenase: 0.1% | PBS | Li RK, Mickle DA, Weisel RD, Zhang J, Mohabeer MK: In vivo survival and function of transplanted rat cardiomyocytes, Circ Res 78, 283-8, 1996 |
| Trypsin: 0.2% | |||||
| 大鼠 | SD, SHR or WKY | Cardiomyocytes | NCIS kit: per instructions | HBSS | MacGregor, R., Klein, R., and Bansal, D.: Secretion of Plasminogen Activator Activity from Neonatal Rat Heart Cells is Regulated by Hormones and Growth Factors, Ann N Y Acad Sci 752, 331, 1995 |
| 大鼠 | 大鼠, SD, male, 200-250 g | Myocytes | Collagenase: 0.07% | Joklik's MEM | Laughlin, M., Schaeffer, M., and Sturek, M.: Effect of Exercise Training on Intracellular Free Ca2+ Transients in Ventricular Myocytes of Rats, Am J Physiol 73, 1141, 1992 |
| Ventricles | |||||
| 大鼠 | 大鼠, SD, female, 60-90 days | Myocardial | Collagenase Type 2: 0.1% | Joklik's MEM, Kreb's-Henseleit buffer, CF | Welder, A.A., Grant, R., Bradlaw, J., and Acosta, D.: A Primary Culture System of Adult Rat Heart Cells for the Study of Toxicologic Agents, In Vitro Cell Dev Biol 27, 921, 1991 |
| 大鼠 | 大鼠, SD, adult, female, 180-220 g | Cardiomyocytes | Collagenase: 0.11% | (see reference) | Fischer, Y., Rose, H., and Kammermeier. H.: Highly Insulin-Responsive Isolated Rat Heart Muscle Cells Yielded By a Modified Isolation Method, Life Sci 49, 1679, 1991 |
| 大鼠 | 大鼠, neonatal, 1-4 days | Cardiomyocytes | Collagenase Type 2: 0.12% | Krebs-Henseleit Buffer, CF | Marino, T.A., Walter, R.,Cobb, E., Palasiuk, M., Parsons, T., and Mercer, W.E.: Effects of Norepinephrine on Neonatal Rat Cardiocyte Growth and Differentiation, In Vitro Cell Dev Biol 26, 229, 1990 |
| 大鼠 | 大鼠 | Heart | Trypsin: 0.06% | 25mM HEPES buffered Locke's solution, CMF | Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and B綿羊 Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990 |
| (also b綿羊) | Adrenal chromaffin | ||||
| Paraneurons | |||||
| 大鼠 | 大鼠, adult, female | Myocytes | Collagenase Type 2: 0.05% | Joklik's MEM | Nag, A.C., Lee, M., and Kosiur, J.R.: Adult Cardiac Muscle Cells in Long-Term Serum-Free Culture:Myofibrillar Organization and Expression of Myosin Heavy Chain Isoforms, In Vitro Cell Dev Biol 26, 464, 1990 |
| 大鼠 | 大鼠s, AW, late-gestation pregnant females | Ventricular | Collagenase Type 2: 0.05 - 0.08% | See reference | Engelmann, G., McTiernan, C., Gerrity, R., and Samarel, A.: Technique 2 (6), 279, 1990 |
| Cardiomyocytes | |||||
| 大鼠 | 大鼠,SD, albino, 10-14 weeks | Myocytes | Collagenase Type 1: 100 u/ml and 150 u/ml | Krebs Ringer bicarbonate buffer | Berg, I., Guse, A.H., and Gercken, G.: Carbamoylcholine-Induced Accumulation of Inositol Mono-,Bis-,Tris-, And Tetrakisphosphates In Isolated Cardiac Myocytes From Adult Rats, Biochim Biophys Acta 1010, 100, 1989 |
| 大鼠 | 大鼠, SD, adult, female, 175 - 225 g | Myocytes | Collagenase: 0.1% | Joklik's medium | Bugaisky, L.B. and Zak, R.: Differentiation of Adult Rat Cardiac Myocytes in Cell Culture, Circ Res 64, 493, 1989 |
| 大鼠 | 大鼠, SD, male, 250 - 350 g | Myocytes, ventricular | Collagenase Type 1: 90 and 100 u/ml | Joklik's solution with and without calcium | De Young, M., Giannattasio, B., and Scarpa, A.: Isolation of Calcium-Tolerant Atrial And Ventricular Myocytes From Adult Rat Heart,Methods Enzymology Vol. 173,, , 662, 1989 |
| 大鼠 | 大鼠, SD, 2-4 day old | Cardiomyocytes | Trypsin: 0.1% | HBSS | Toraason, M., Luken, M., Breitenstein, M., Krueger, J., and Biagini, R.:Comparative Toxicity of Allylamine and Acrolein in Cultured Myocytes and Fibroblasts from Neonatal Rat Heart, Toxicology 56, 107, 1989 |
| Fibroblasts | |||||
| 大鼠 | 大鼠, SD, male | Aortic smooth muscle | Trypsin: 0.05% | DMEM | Cornwell, T., and Lincoln, T.: Regulation of Intracellular Ca2+ Levels in Cultured Vascular Smooth Muscle Cells, J Biol Chem 264 (2), 1146, 1989 |
| 大鼠 | 大鼠, adult, 150-200 g | Myocytes, atria | Collagenase Type 1: 100 u/ml | Kreb's Ringer bicarbonate - HEPES buffer | McMahon, K.K.: A Study of Adult Rat Atrial Myocyte Attachment to Extricellular Matrix Components and Long Term Culture,Biology of Isolated Adult Cardiac Myocytes,(Clark, Decker, Borg, Eds), Elsevier Science Publishing Co. Inc., 318, 1988 |
| 大鼠 | SD大鼠 | Myocytes | Collagenase Type 2: 0.1% | Kreb's Ringer w/ calcium | Buxton, I.L.O. and Doggwiler, K.O.: Alpha1-Adrenergic Receptor Signal Transduction in the Adult Rat Cardiac Myocyte,Biology of Isolated Adult Cardiac Myocytes,(Clark, Decker, Borg, eds), , 298, 1988 |
| 大鼠 | SD大鼠, 雄性, 250-350 克 | Myocytes, heart | Hyaluronidase: 0.03% | Bicarbonate-buffered medium | Kim, D. and Smith, T.W.: Temporal Variation in Contractile State And [Ca++] in Isolated Adult Rat And Guinea Pig Cardiac Myocytes,Biology of Isolated Adult Cardiac Myocytes,(Clark, Decker, Borg, eds), Elsevier Science Publishing Co. Inc., 370, 1988 |
| 大鼠 | Wistar大鼠, 2-4 天neonates | Myoblast, cardiac | Trypsin: 0.1% | Standardized Medium | Grynberg, A., Degois, M., Guenot, L., and Athias, P.: Primary Rat Cardiac Cell Culture: Diet of the Mother Rats as a Determinant Parameter of Cardiomyoblast Production from Neonates, Biol Cell 57, 89, 1986 |
| (see reference) | |||||
| 大鼠 | 大鼠, SD, 懷孕的 | Myocytes | Trypsin: 2.4 u/ml | DMEM | Freerksen, D., Schroedl, N., and Hartzell, C.: Control of Enzyme Activity Levels by Serum and Hydrocortisone in Neonatal Rat Heart Cells Cultured in Serum-Free Medium, J Cell Physiol 120, 126, 1984 |
| 大鼠 | 大鼠, SD, 雌性, 12 周 | Myocytes | Collagenase Type 1: 0.06% | Krebs Ringer bicarbonate buffer | Piper, H.M., Probst, I., Schwartz, P., Hutter, F.J., and Spieckermann, P.G.:Culturing of Calcium Stable Adult Cardiac Myocytes, J Mol Cell Cardiol 14, 397, 1982 |
| 大鼠 | 大鼠, 雄性/雌性, 200-250 克 | Muscle | Hyaluronidase: 0.1% | Krebs Ringer phosphate buffer, CMF | Nag, A. and Cheng, M.: Adult Mammalian Cardiac Muscle Cells in Culture,Tissue Cell 13, 515, 1981 |
| 大鼠 | 大鼠, 雌性, 成年, 200 - 300 克 | Myocytes | Collagenase Type 2: 0.1% | Joklik's MEM | Claycomb, W.C., and Palazzo, M.C.: Culture of The Terminally Differentiated Adult Cardiac Muscle Cell: A Light and Scanning Electron Microscope Study,Dev Biol 80, 466, 1980 |
| 大鼠 | SD大鼠, 成年, 雄性, 200-250 克 | Myocytes | Hyaluronidase: 0.10% | Joklik MEM, CF | Frangakis, C., Bahl, J., McDaniel, H., and Bressler, R.: Tolerance to Physiological Calcium by Isolated Mycocytes from the Adult Rat Heart; An Improved Cellular Preparation, Life Sci 27, 815, 1980 |
| 大鼠 | SD大鼠, 雌性, 12 周, 250 克 | Myocytes | Collagenase Type 1: 0.20% | Kreb's Ringer bicarbonate buffer | Powell, T., Terrar, D.A., and Twist, V.W.: Electrical Properties of Individual Cells Isolated From Adult Rat Ventricular Myocardium, J Physiol 302, 131, 1980 |
| Ventricular myocardium | |||||
| 大鼠 | 大鼠, 10 周 | Myocytes | Collagenase Type 1: 0.1% | Saline | Bishop, S., Oparil, S., Reynolds, R., and Drummond, J.: Regional Myocyte Size in Normotensive and Spontaneously Hypertensive Rats, Hypertension 1 (4), 378, 1979 |
| 大鼠 | 大鼠, 雄性, 200-300 克 | Myocytes | Hyaluronidase: 82 u/ml | Kreb's Henseleit buffer | Farmer, B., Harris, R., Jolly, W., Hathaway, D., Katzberg, A., Watanabe, A., Whitlow, A., and Besch, H.: Isolation and Characterization of Adult Rat Heart Cells, Arch Biochem Biophys 179, 545, 1977 |
| 大鼠 | 大鼠,雌性, 白化變種, 200-250克 | Myocytes | Hyaluronidase: 0.1% | Kreb's Ringers phosphate | Nag, A., Fischman, D., Aumont, M., and Zak, R.: Studies of Isolated Adult Rat Heart Cells: The Surface Morphology and the Influence of Extracellular Calcium Ion Concentration on Cellular Viability, Tissue Cell 9 (3), 419, 1977 |
| 大鼠 | 大鼠, 成年, 雄性, 150-200克 | Myocytes | Collagenase: 0.1% | MEM CF | Grosso, D., Frangakis, C., Carlson, E., and Bressler, R.: Isolation and Characterization of Myocytes From the Adult Rat Heart, Prep Biochem 7 (5), 383, 1977 |
| Hyaluronidase: 0.1% | |||||
| 大鼠 | 大鼠, 成年的 | Myocytes | Collagenase: 0.1% | Perfusing solution | Moustafa, E., Skomedal, T., Osnes, J., and Oye, I.: Cyclic Amp Formation and Morphology of Myocardial Cells Isolated from Adult Heart: Effect of Ca2+ and Mg2+, Biochim Biophys Acta 421, 411, 1976 |
| 大鼠 | SD大鼠, 白化變種, 雄性, 180-230 克 | Heart ventricles, beating | Collagenase: 0.05-0.2% | Phosphate buffer | Glick, M., Burns, A., and Reddy, W.: Dispersion and Isolation of Beating Cells from Adult Rat Heart, Anal Biochem 61, 32, 1974 |
| 大鼠 | 大鼠, 希伯來(lái)聯(lián)合國(guó), 3 & 17 個(gè)月, 150-210 克 | Myocytes | Trypsin: 0.05% | Versene buffer | Bierman, E.L., Stein, O., and Stein, Y.: Lipoprotein Uptake and Metabolism by Rat Aortic Smooth Muscle Cells in Tissue Culture, Circ Res 35, 136, 1974 |
| 大鼠 | 大鼠, 2-5 天 | Myocytes | Trypsin: 0.01% | Saline A | Speicher, D., and McCarl, R.: Pancreatic Enzyme Requirements for the Dissociation of Rat Hearts for Culture, In Vitro 10, 30, 1974 |
| 大鼠 | 大鼠, SD, male, 150-200克 | Myocytes | Hyaluronidase: 0.05% | (see reference) | Pretlow II, T., Glick, M., and Reddy, W.: Separation of Beating Cardiac Myocytes from Suspensions of Heart Cells, Am J Pathol 67 (2), 215, 1972 |
| 大鼠 | 大鼠, albino, male, 250 克 | Myocytes | Hyaluronidase: 0.20% | Hank's solution, CF | Berry, M., Friend, D., and Scheuer, J.: Morphology and Metabolism of Intact Muscle Cells Isolated from Adult Rat Heart, Circ Res 26, 679, 1970 |
| 大鼠 | 大鼠, 成年的, 雄性, 250 克 | Myocytes | Trypsin: 0.1% | Saline A | Vahouny, G.V., Wei, R., Starkweather, R., and Davis, C.: Preparation of Beating Heart Cells From Adult Rats, Science 167, 1616, 1970 |
| 大鼠 | Wistar大鼠, 1-4 天大 | Myocytes | Trypsin: 0.125% | Gey's BSS | Mark, G., and Strasser, F.: Pacemaker Activity and Mitosis in Cultures of Newborn Rat Heart Ventricle Cells, Exp Cell Res 44, 217, 1966 |
| 貝類動(dòng)物 | 太平洋牡蠣 | Haemocytes | Trypsin: | L15 medium | Le Duff, R., Lipart, C., and Renault, T.: Primary Culture of Pacific Oyster, Crassostrea gigas, heart cells, J Tiss Cul Meth 16, 67, 1994 |
| 貝類動(dòng)物 | 蚌, 3.0 - 4.5 厘米 | Myocytes | Collagenase Type 1: 2% | L15 medium | Wen, C., Kou, G., and Chen, S.: C*tion of Cells From the Heart of the Hard Clam, Merretrix lusoria (Roding), J Tiss Cul Meth 15, 123, 1993 |
| Fibroblasts |
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